Počet záznamů: 1  

Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage

  1. 1.
    SYSNO ASEP0375448
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevScreening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage
    Tvůrce(i) Amps, K. (GB)
    Andrews, P.W. (GB)
    Anyfantis, G. (GB)
    Armstrong, L. (GB)
    Avery, S. (SG)
    Baharvand, H. (IR)
    Baker, J. (US)
    Baker, D. (GB)
    Munoz, M. N. (GB)
    Beil, S. (US)
    Benvenisty, N. (IL)
    Ben-Yosef, D. (IL)
    Biancotti, J. C. (US)
    Bosman, A. (CH)
    Brena, R. M. (US)
    Brison, D. (GB)
    Caisander, G. (SE)
    Camarasa, M. V. (GB)
    Chen, J. M. (SG)
    Chiao, E. (US)
    Choi, Y. M. (KP)
    Choo, E. (SG)
    Collins, D. (GB)
    Colman, A. (SG)
    Crook, J. M. (SG)
    Daley, G. Q. (US)
    Dalton, A. (GB)
    De Sousa, P. A. (GB)
    Denning, C. (GB)
    Downie, J. (GB)
    Dvořák, P. (CZ)
    Hampl, Aleš (UEM-P)
    Celkový počet autorů125
    Zdroj.dok.Nature Biotechnology. - : Nature Publishing Group - ISSN 1087-0156
    Roč. 29, č. 12 (2011), s. 1132-1144
    Poč.str.13 s.
    Jazyk dok.eng - angličtina
    Země vyd.GB - Velká Británie
    Klíč. slovacomparative genomic hybridization ; copy number variation ; human es cells
    Vědní obor RIVEB - Genetika a molekulární biologie
    CEZAV0Z50390703 - UEM-P (2007-2013)
    UT WOS000298038700025
    DOI10.1038/nbt.2051
    AnotaceThe International Stem Cell Initiative analyzed 125 human embryonic stem (ES) cell lines and 11 induced pluripotent stem (iPS) cell lines, from 38 laboratories worldwide, for genetic changes occurring during culture. Most lines were analyzed at an early and late passage. Single-nucleotide polymorphism (SNP) analysis revealed that they included representatives of most major ethnic groups. Most lines remained karyotypically normal, but there was a progressive tendency to acquire changes on prolonged culture, commonly affecting chromosomes 1, 12, 17 and 20. DNA methylation patterns changed haphazardly with no link to time in culture. Structural variants, determined from the SNP arrays, also appeared sporadically. No common variants related to culture were observed on chromosomes 1, 12 and 17, but a minimal amplicon in chromosome 20q11.21, including three genes expressed in human ES cells, ID1, BCL2L1 and HM13, occurred in >20% of the lines. Of these genes, BCL2L1 is a strong candidate for driving culture adaptation of ES cells.
    PracovištěÚstav experimentální medicíny
    KontaktLenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218
    Rok sběru2012
Počet záznamů: 1  

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