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Preconceptional paternal glycidamide exposure affects embryonic gene expression: Single embryo gene expression study following in vitro fertilization

SYSNO ASEP	0371924
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Preconceptional paternal glycidamide exposure affects embryonic gene expression: Single embryo gene expression study following in vitro fertilization
Tvůrce(i)	Brevik, A.. (NO) Rusňáková, Vendula (BTO-N) Duale, N. (NO) Slagsvold, H.H. (NO) Olsen, A.-K. (NO) Storeng, R. (NO) Kubista, Mikael (BTO-N)_RID Brunborg, G. (NO) Lindeman, B. (NO)
Zdroj.dok.	Reproductive Toxicology. - : Elsevier - ISSN 0890-6238 Roč. 32, č. 4 (2011), s. 463-471
Poč.str.	9 s.
Jazyk dok.	eng - angličtina
Země vyd.	NO - Norsko
Klíč. slova	Single-cell gene expression ; Glycidamide ; Acrylamide
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	IAA500520809 GA AV ČR - Akademie věd
CEZ	AV0Z50520701 - BTO-N (2007-2013)
UT WOS	000297716900010
DOI	10.1016/j.reprotox.2011.09.005
Anotace	Recognition of early determinants of disease onset has sparked an interest in paternally transmitted factors and their impact on the developing embryo. Acrylamide (AA),a widely distributed xenobiotic compound,is converted to its active metabolite glycidamide (GA) by the CYP2E1 enzyme.Based on its capacity to induce dominant lethal mutations,we hypothesized that paternal GA exposure would have a negative impact on embryonic genome activation,via GA-DNA and protamine adducts persisting in the fertilizing sperm. Using a combination of in vitro fertilization (IVF) techniques and RT-qPCR single embryo gene expression (SEGE), we studied the expression of key DNA repair genes and genes important for embryo development, at the 1-, 2-, 4- and 8-cell stage of the developing mouse embryo. Compared to controls paternal GA-exposure gave rise to an altered pattern of embryonic gene expression, with an initial reduced expression at early stages followed by increased expression at the 8-cell stage.
Pracoviště	Biotechnologický ústav
Kontakt	Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700
Rok sběru	2012

Počet záznamů: 1