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Wound healing gene therapy: cartilage regeneration induced by vascular endothelial growth factor plasmid
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SYSNO ASEP 0370725 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Wound healing gene therapy: cartilage regeneration induced by vascular endothelial growth factor plasmid Tvůrce(i) Kološtová, K. (CZ)
Taltynov, O. (CZ)
Pintérová, D. (CZ)
Boubelík, M. (CZ)
Raška, O. (CZ)
Hozák, Pavel (UMG-J) RID, ORCID
Jirkovská, M. (CZ)
Bobek, V. (CZ)Zdroj.dok. American Journal of Otolaryngology. - : Elsevier - ISSN 0196-0709
Roč. 33, č. 1 (2012), s. 68-74Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova BALB/c mouse strain ; significant angiogenesis ; cartilage repair ; phVEGF(165) injection Vědní obor RIV EB - Genetika a molekulární biologie CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000298070800012 DOI 10.1016/j.amjoto.2011.01.005 Anotace Vascular endothelial growth factor (VEGF) is the most critical regulator of vascular formation. Using plasmid transfer of human complementary DNA VEGF165 (phVEGF165) we performed therapeutic angiogenesis in experimental skin and cartilage trauma in BALB/c mice. At 6 weeks of age all mice were ear-punched and phVEGF165 injected into the first ear and vector without insert or saline injection into the second one. The healing process was followed. The hollow diameter was measured and histological sections of experimental and control pinnae were taken regularly after experimental injury for hematoxylin and eosin and periodic acid Schiff staining and for human VEGF immunocytochemistry. Human VEGF expression was also checked by real-time PCR in formalin-fixed, paraffin-embedded tissue sections. Significant angiogenesis promotion and cartilage repair were observed in the mice. Administering phVEGF165 thus leads to faster cartilage regeneration even if not only on the angiogenic basis. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2012
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