Počet záznamů: 1
Antibody repertoire development in fetal and neonatal piglets. XX. B cell lymphogenesis is absent in the ileal Peyer´s patches, their repertoire development is antigen dependent, and they are not required for B cell maintenance
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SYSNO ASEP 0369862 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Antibody repertoire development in fetal and neonatal piglets. XX. B cell lymphogenesis is absent in the ileal Peyer´s patches, their repertoire development is antigen dependent, and they are not required for B cell maintenance Tvůrce(i) Butler, J. (US)
Santiago-Mateo, K. (US)
Sun, X.-Z. (US)
Wertz, N. (US)
Šinkora, Marek (MBU-M) RID, ORCID
Francis, D. (US)Zdroj.dok. Journal of Immunology. - : American Association of Immunologists - ISSN 0022-1767
Roč. 187, č. 10 (2011), s. 5141-5149Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova neonatal piglets ; Peyer’s patches ; b cell Vědní obor RIV EC - Imunologie CEP ME09089 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000296767300023 DOI 10.4049/jimmunol.1101871 Anotace The continuous ileal Peyer’s patches (IPP) of sheep are regarded as a type of mammalian bursal equivalent where B cells diversify their repertoire in an Ag-independent fashion. Anatomically and developmentally similar IPP occur in swine. Resection of ~90% of the IPP in piglets at birth did not alter Ig levels in serum and secretions or retard diversification of the Ab repertoire when animals were maintained in isolators and colonized with a defined gut flora. Resection or sham surgery elevated IgG and IgA in serum and in lavage fluid from the gut, lung, and in saliva. No changes in the frequency of IgG-, IgA-, and IgM-containing cells in the spleen and peripheral lymph node were observed. Using an index that quantifies diversification of the VDJ repertoire, no differences were seen in three secondary lymphoid tissues between piglets lacking IPP and colonized controls, whereas both groups displayed >10-fold greater diversification than did late-term fetal piglets or piglets maintained germ-free. Somatic hypermutation was very low in fetal IPP and the IPP of germ-free piglets but increased 3- to 5-fold after colonization. D–J signal joint circles were not recovered in IPP, and V–DJ signal joint circles were 5-fold lower than in bone marrow and similar to those in thymus and spleen Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2012
Počet záznamů: 1