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Asbestos exposure affects poly(ADP-ribose) polymerase-1 activity: role in asbestos-induced carcinogenesis
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SYSNO ASEP 0369504 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Asbestos exposure affects poly(ADP-ribose) polymerase-1 activity: role in asbestos-induced carcinogenesis Tvůrce(i) Tomasetti, M. (IT)
Amati, M. (IT)
Nocchi, L. (IT)
Saccucci, F. (IT)
Strafella, E. (IT)
Tarquini, L.M. (IT)
Carbonari, D. (IT)
Alleva, R. (IT)
Borghi, B. (IT)
Neužil, Jiří (BTO-N) RID
Bracci, M. (IT)
Santarelli, L. (IT)Zdroj.dok. MUTAGENESIS - ISSN 0267-8357
Roč. 26, č. 5 (2011), s. 585-591Poč.str. 7 Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Asbestos exposure ; poly(ADP-ribose) polymerase-1 ; malignant mesothelioma Vědní obor RIV EB - Genetika a molekulární biologie CEZ AV0Z50520701 - BTO-N (2007-2013) UT WOS 000294554600002 DOI 10.1093/mutage/ger020 Anotace Asbestos is known to induce malignant mesothelioma (MM). It is directly genotoxic by inducing DNA strand breaks. Poly(ADP-ribose) polymerase-1 (PARP1) is a protein with a function as a DNA damage sensor. To determine whether PARP1 is involved in asbestos-induced carcinogenesis, PARP1 expression and activity as well as DNA damage and repair were evaluated in circulating cells of asbestos-exposed subjects, MM patients and controls. Accumulation of the pre-mutagenic 8-hydroxy-2'-deoxyguanosine and elevated PARP1 expression were found both in asbestos-exposed subjects and MM patients. Although PARP1 was highly expressed, its activity was relatively low in circulating cells as well as in MM biopsies. Low DNA repair efficiency was observed in lymphocytes from MM patients. To mimic PARP1 dysfunction, PARP1 expression and activity were induced in immortalised mesothelial cells by their exposure to asbestos in the presence of a PARP1 inhibitor, which resulted in transformation of the cells. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2012
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