Počet záznamů: 1  

Matrix-Assisted Laser Desorption Ionization (MALDI)-Time of Flight Mass Spectrometry- and MALDI Biotyper-Based Identification of Cultured Biphenyl-Metabolizing Bacteria from Contaminated Horseradish Rhizosphere Soil

  1. 1.
    SYSNO ASEP0368927
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevMatrix-Assisted Laser Desorption Ionization (MALDI)-Time of Flight Mass Spectrometry- and MALDI Biotyper-Based Identification of Cultured Biphenyl-Metabolizing Bacteria from Contaminated Horseradish Rhizosphere Soil
    Tvůrce(i) Uhlík, Ondřej (UOCHB-X)
    Strejček, M. (CZ)
    Junková, P. (CZ)
    Šanda, Miloslav (UOCHB-X)
    Hroudová, Miluše (UMG-J) RID
    Vlček, Čestmír (UMG-J) RID
    Macková, Martina (UOCHB-X)
    Macek, Tomáš (UOCHB-X)
    Celkový počet autorů8
    Zdroj.dok.Applied and Environmental Microbiology. - : American Society for Microbiology - ISSN 0099-2240
    Roč. 77, č. 19 (2011), s. 6858-6866
    Poč.str.9 s.
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovaMALDI-TOF MS ; bioremediation ; MALDI Biotyper ; bacterial identification
    Vědní obor RIVCC - Organická chemie
    CEZAV0Z40550506 - UOCHB-X (2005-2011)
    AV0Z50520514 - UMG-J (2005-2011)
    UT WOS000295123300016
    DOI10.1128/AEM.05465-11
    AnotaceBacteria that are able to utilize biphenyl as a sole source of carbon were extracted and isolated from polychlorinated biphenyl (PCB)-contaminated soil vegetated by horseradish. Isolates were identified using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The usage of MALDI Biotyper for the classification of isolates was evaluated and compared to 16S rRNA gene sequence analysis. A wide spectrum of bacteria was isolated, with Arthrobacter, Serratia, Rhodococcus, and Rhizobium being predominant. Arthrobacter isolates also represented the most diverse group. The use of MALDI Biotyper in many cases permitted the identification at the level of species, which was not achieved by 16S rRNA gene sequence analyses. However, some isolates had to be identified by 16S rRNA gene analyses if MALDI Biotyper-based identification was at the level of probable or not reliable identification, usually due to a lack of reference spectra included in the database. Overall, this study shows the possibility of using MALDI-TOF MS and MALDI Biotyper for the fast and relatively nonlaborious identification/classification of soil isolates. At the same time, it demonstrates the dominant role of employing 16S rRNA gene analyses for the identification of recently isolated strains that can later fill the gaps in the protein-based identification databases.
    PracovištěÚstav organické chemie a biochemie
    Kontaktasep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
    Rok sběru2012
Počet záznamů: 1  

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