Počet záznamů: 1
A non-radioactive assay for precise determination of intracellular levels of imatinib and its main metabolite in Bcr-Abl positive cells
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SYSNO ASEP 0368600 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název A non-radioactive assay for precise determination of intracellular levels of imatinib and its main metabolite in Bcr-Abl positive cells Tvůrce(i) Mlejnek, P. (CZ)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Doležel, P. (CZ)Celkový počet autorů 3 Zdroj.dok. Talanta. - : Elsevier - ISSN 0039-9140
Roč. 83, č. 5 (2011), s. 1466-1471Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova K562 cells ; P-glycoprotein ; Multidrug resistance ; N-desmethyl imatinib ; CGP 74588 Vědní obor RIV EF - Botanika CEP GA301/08/1649 GA ČR - Grantová agentura ČR CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000287110100023 DOI 10.1016/j.talanta.2010.11.028 Anotace Multidrug resistance (MDR) is often associated with overexpression of the P-glycoprotein (P-gp, ABCB1). It was demonstrated that the P-gp mediated efflux decreases the drug concentration in cancer cells which results in the failure of chemotherapy. However, the MDR phenotype in cancer cells obviously involves various mechanisms. Therefore, if we want to estimate a contribution of the P-gp expression to the MDR phenotype, a clear quantitative relationship between the intracellular drug level and cell sensitivity must be established. To achieve this goal, a sensitive and non-radioactive assay for precise determination of intracellular levels of imatinib and its main metabolite N-desmethyl imatinib (CGP 74588) has been developed. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2012
Počet záznamů: 1