Počet záznamů: 1
Stable transformation of Mesembryanthemum crystallinum (L.) with Agrobacterium rhizogenes harboring the green fluorescent protein targeted to the endoplasmic reticulum
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SYSNO ASEP 0368594 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Stable transformation of Mesembryanthemum crystallinum (L.) with Agrobacterium rhizogenes harboring the green fluorescent protein targeted to the endoplasmic reticulum Tvůrce(i) Konieczny, R. (PL)
Obert, B. (SK)
Bleho, J. (SK)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Heym, C. (DE)
Tuleja, M. (PL)
Mueller, J. (DE)
Strnad, Miroslav (UEB-Q) RID, ORCID
Menzel, D. (DE)
Šamaj, J. (CZ)Celkový počet autorů 10 Zdroj.dok. Journal of Plant Physiology. - : Elsevier - ISSN 0176-1617
Roč. 168, č. 7 (2011), s. 722-729Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. PT - Portugalsko Klíč. slova Agrobacterium ; Callus ; Common ice plant ; Cytokinin content ; GFP Vědní obor RIV EF - Botanika CEP GA301/08/1649 GA ČR - Grantová agentura ČR ED0007/01/01 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000290058600013 DOI 10.1016/j.jplph.2010.10.013 Anotace Stable transformation of Mesembryanthemum crystallinum L (common ice plant) with a green fluorescent protein (GFP) construct targeted to the endoplasmic reticulum was obtained. Seven and fourteen days after germination seedlings were infected with Agro bacterium rhizogenes strain ARqua1 either by direct coating of the cut radicles with bacteria growing on solid medium or by immersion of the cut surface in bacterial suspension at different optical densities. Both methods of infection resulted in production of GFP-positive roots with a frequency ranging from 6 to 20% according to the age of the explants and the application procedure. The green fluorescing roots displayed the typical hairy root phenotype and were easily maintained in liquid medium without growth regulators for over 2 years. Stable expression of the transgene in the roots was confirmed by polymerase chain reaction (PCR), immunoblotting and the capacity of roots to grow and produce callus on kanamycin-enriched medium. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2012
Počet záznamů: 1