Počet záznamů: 1

Analysis of Marker Expression in Porcine Cell Lines Derived from Blastocysts Produced In Vitro and In Vivo

SYSNO ASEP	0368327
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Analysis of Marker Expression in Porcine Cell Lines Derived from Blastocysts Produced In Vitro and In Vivo
Tvůrce(i)	Vacková, I. (CZ) Nováková, Z. (CZ) Krylov, V. (CZ) Okada, K. (JP) Kott, T. (CZ) Fulka, H. (CZ) Motlík, Jan (UZFG-Y)_{RID, ORCID}
Celkový počet autorů	7
Zdroj.dok.	Journal of Reproduction and Development. - : Society for Reproduction and Development - ISSN 0916-8818 Roč. 57, č. 5 (2011), s. 594-603
Poč.str.	10 s.
Jazyk dok.	eng - angličtina
Země vyd.	JP - Japonsko
Klíč. slova	Blastocyst ; In vivo and in vitro ; Pig ; Stem cells
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	QI101A166 GA MZe - Ministerstvo zemědělství
	1M0538 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
CEZ	AV0Z50450515 - UZFG-Y (2005-2011)
UT WOS	000296597100006
DOI	10.1262/jrd.10-184H
Anotace	The present study was designed to extensively characterize cell lines derived from porcine blastocysts by several methodical approaches, including morphological observation, cytogenetic analysis, estimation of alkaline phosphatase activity and detection of specific marker expression at the mRNA/protein level. A comparison was made between the properties of cell lines isolated from in vivo- and in vitro-obtained blastocysts. Our results showed that 57.1% of the in vivo-obtained blastocysts attached to the feeder layer and that 33.3% of them started to grow in a monolayer. The percentage of attached in vitro-produced blastocysts was lower (24.6%), and only 6.9% of them started to grow. Outgrowths from the in vitro-produced blastocysts formed mainly trophectoderm or epithelial-like monolayer, whereas the in vivo-obtained blastocysts formed heterogeneous outgrowths that also contained cells with embryonic stem (ES)-like morphology.
Pracoviště	Ústav živočišné fyziologie a genetiky
Kontakt	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Rok sběru	2012

Počet záznamů: 1