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Human Embryonic Stem Cells Suffer from Centrosomal Amplification

SYSNO ASEP	0367964
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Human Embryonic Stem Cells Suffer from Centrosomal Amplification
Tvůrce(i)	Holubcová, Z. (CZ) Matula, P. (CZ) Sedláčková, M. (CZ) Vinarský, Vladimír (UEM-P) Doležalová, Dáša (UEM-P) Bárta, Tomáš (UEM-P) Dvořák, Petr (UEM-P) Hampl, Aleš (UEM-P)
Zdroj.dok.	Stem Cells. - : Oxford University Press - ISSN 1066-5099 Roč. 29, č. 1 (2011), s. 46-56
Poč.str.	11 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	human embryonic stem cells ; centrosome ; chromosome
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	GA204/09/2044 GA ČR - Grantová agentura ČR
CEZ	AV0Z50390703 - UEM-P (2007-2013)
UT WOS	000286659300007
DOI	10.1002/stem.549
Anotace	Propagation of human embryonic stem cells (hESCs) in culture tends to alter karyotype, potentially limiting the prospective use of these cells in patients. The chromosomal instability of some malignancies is considered to be driven, at least in part, by centrosomal overamplification, perturbing balanced chromosome segregation. Here, we report, for the first time, that very high percentage of cultured hESCs has supernumerary centrosomes during mitosis. Supernumerary centrosomes were strictly associated with an undifferentiated hESC state and progressively disappeared on prolonged propagation in culture. Improved attachment to culture substratum and inhibition of CDK2 and Aurora A (key regulators of centrosomal metabolism) diminished the frequency of multicentrosomal mitoses. Collectively, our data indicate that supernumerary centrosomes are a significant risk factor for chromosome instability in cultured hESCs and should be evaluated when new culture conditions are being implemented.
Pracoviště	Ústav experimentální medicíny
Kontakt	Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218
Rok sběru	2012

Počet záznamů: 1