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Applications of phasors to in vitro time-resolved fluorescence measurements
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SYSNO ASEP 0367710 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Applications of phasors to in vitro time-resolved fluorescence measurements Tvůrce(i) Štefl, Martin (UFCH-W)
James, N. G. (US)
Jameson, D. M. (US)Zdroj.dok. Analytical Biochemistry. - : Elsevier - ISSN 0003-2697
Roč. 410, č. 1 (2011), s. 62-69Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova phasor ; fraquency domain ; fluorescence lifetimes Vědní obor RIV CF - Fyzikální chemie a teoretická chemie CEP LC06063 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z40400503 - UFCH-W (2005-2011) UT WOS 000286711300010 DOI 10.1016/j.ab.2010.11.010 Anotace The phasor method of treating fluorescence lifetime data provides a facile and convenient approach to characterize lifetime heterogeneity and to detect the presence of excited state reactions such as solvent relaxation and Forster resonance energy transfer. The method uses a plot of M sin(Phi) versus M cos(Phi), where M is the modulation ratio and Phi is the phase angle taken from frequency domain fluorometry. A principal advantage of the phasor method is that it provides a model-less approach to time-resolved data amenable to visual inspection. Although the phasor approach has been recently applied to fluorescence lifetime imaging microscopy, it has not been used extensively for cuvette studies. In the current study, we explore the applications of the method to in vitro samples. The phasors of binary and ternary mixtures of fluorescent dyes demonstrate the utility of the method for investigating complex mixtures. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2012
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