Počet záznamů: 1

Reproductive tissue expression and sperm localization of porcine beta-microseminoprotein

SYSNO ASEP	0367617
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Reproductive tissue expression and sperm localization of porcine beta-microseminoprotein
Tvůrce(i)	Maňásková-Postlerová, Pavla (BTO-N)_ORCID Davidová, Nina (BTO-N) Šulc, Miroslav (MBU-M)_{RID, ORCID} Philimonenko, Anatoly (UMG-J) Hozák, Pavel (UMG-J)_{RID, ORCID} Jonáková, Věra (BTO-N)_RID
Celkový počet autorů	6
Zdroj.dok.	Cell and Tissue Research - ISSN 0302-766X Roč. 344, č. 2 (2011), s. 341-353
Poč.str.	13 s.
Jazyk dok.	eng - angličtina
Země vyd.	DE - Německo
Klíč. slova	porcine beta-microseminoprotein ; reproductive organs ; spermatozoa ; immunofluorescence ; sperm acrosome ; boar
Vědní obor RIV	CE - Biochemie
CEP	GA303/09/1285 GA ČR - Grantová agentura ČR
	GD523/08/H064 GA ČR - Grantová agentura ČR
	NS10009 GA MZd - Ministerstvo zdravotnictví
	1M06011 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	LC07017 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	LC545 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
CEZ	AV0Z50520701 - BTO-N (2007-2013)
	AV0Z50520514 - UMG-J (2005-2011)
	AV0Z50200510 - MBU-M (2005-2011)
UT WOS	000290167600016
DOI	10.1007/s00441-011-1149-y
Anotace	Porcine beta-microseminoprotein (MSP) has been described as a protein characteristic of the prostate; however, we proved its mRNA transcripts in all reproductive organs. In tissue extracts, specific antibody antiMSP recognized a 12-kDa protein band, which was identified by mass spectrometry as MSP, and immunofluorescence displayed the MSP occurrence in epithelial tissue. MSP was localized on epididymal sperm in the acrosomal region and in addition to that on the flagellum of ejaculated sperm. The MSP absence on the surface of capacitated sperm together with the antibody detection of MSP in the sperm acidic extract after in vitro capacitation indicated its acrosomal origin. The MSP sperm localization was confirmed by electron microscopy. The secretion of porcine MSP by different reproductive organs might indicate its multiple roles in the reproductive process, and for the first time in mammalian species, the MSP localization has been shown at different physiological stages of sperm.
Pracoviště	Biotechnologický ústav
Kontakt	Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700
Rok sběru	2012

Počet záznamů: 1