Počet záznamů: 1

Application of Colloidal Palladium Nanoparticles for Labeling in Electron Microscopy

SYSNO ASEP	0367418
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Application of Colloidal Palladium Nanoparticles for Labeling in Electron Microscopy
Tvůrce(i)	Vancová, Marie (BC-A)_{RID, ORCID} Šlouf, Miroslav (UMCH-V)_{RID, ORCID} Langhans, Jan (BC-A) Pavlová, Eva (UMCH-V)_RID Nebesářová, Jana (BC-A)_{RID, ORCID}
Zdroj.dok.	Microscopy and Microanalysis. - : Cambridge University Press - ISSN 1431-9276 Roč. 17, č. 5 (2011), s. 810-816
Poč.str.	7 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	electron microscopy ; colloidal palladium ; nanoparticles ; labeling ; salivary glands ; Ixodes ricinus
Vědní obor RIV	CF - Fyzikální chemie a teoretická chemie
CEP	KAN200520704 GA AV ČR - Akademie věd
	KJB600960906 GA AV ČR - Akademie věd
	GAP205/10/0348 GA ČR - Grantová agentura ČR
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
	AV0Z40500505 - UMCH-V (2005-2011)
UT WOS	000295609100020
DOI	10.1017/S1431927611000547
Anotace	The application of palladium nanoparticles as electron-dense markers for labeling in both transmission and scanning electron microscopy requires their conjugation to a specific protein. The conjugation protocol described here includes the dihydrolipoic acid capping of Pd nanoparticles and their subsequent covalent attachment to protein molecules such as streptavidin, protein A, or avidin. The single-step reaction was mediated using the cross-linking agent ethyl-3-(3- dimethylaminopropyl)carbodiimide. The final Pd conjugates were fully functional, as demonstrated by labeling of ultrathin resin sections of either bovine serum albumin or secretory granules of the salivary gland isolated from the partially fed female Ixodes ricinus tick. The results were quantified, evaluated, and compared with results obtained using commercially available gold particle conjugates. Both the streptavidin-Pd conjugate and commercial streptavidin-Au conjugate were used to detect fucosylated glycans.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2012

Počet záznamů: 1