Počet záznamů: 1
Complex modulation of peptidolytic activity of cathepsin D by sphingolipids
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SYSNO ASEP 0366668 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Complex modulation of peptidolytic activity of cathepsin D by sphingolipids Tvůrce(i) Žebrakovská, Iva (UOCHB-X) ORCID, RID
Máša, Martin (UOCHB-X)
Srp, Jaroslav (UOCHB-X) RID, ORCID
Horn, Martin (UOCHB-X) RID, ORCID
Vávrová, K. (CZ)
Mareš, Michael (UOCHB-X) RID, ORCIDCelkový počet autorů 6 Zdroj.dok. Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids. - : Elsevier - ISSN 1388-1981
Roč. 1811, č. 12 (2011), s. 1097-1104Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova sphingolipid ; phospholipid ; inhibition ; activation ; cathepsin D ; enzyme regulation Vědní obor RIV CE - Biochemie CEP IAA400550705 GA AV ČR - Akademie věd CEZ AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000298521700012 DOI 10.1016/j.bbalip.2011.09.005 Anotace Cathepsin D is an aspartic peptidase involved in cellular processes including proliferation and apoptosis. We describe a complex pattern of modulation of the peptidolytic activity of cathepsin D by sphingolipids. A panel of sphingolipid derivatives was screened in a FRET-based assay; these molecules demonstrated negative or positive modulation of cathepsin D peptidolytic activity, depending on the sphingolipid structure. Certain sphingosines and ceramides inhibited cathepsin D, and structural requirements for this inhibitory effect were evaluated. In contrast, monoester phosphosphingolipids, especially ceramide-1-phosphate, were identified as activators of cathepsin D peptidolytic activity Thus, sphingolipids and phosphosphingolipids, known to be antagonistic in their cell-signaling functions, displayed opposite modulation of cathepsin D. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Rok sběru 2012
Počet záznamů: 1