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Impact of homogenization and protein extraction conditions on the obtained tobacco pollen proteomic patterns
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SYSNO ASEP 0366403 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Impact of homogenization and protein extraction conditions on the obtained tobacco pollen proteomic patterns Tvůrce(i) Fíla, Jan (UEB-Q) RID, ORCID
Čapková, Věra (UEB-Q) RID
Feciková, Jana (UEB-Q) ORCID
Honys, David (UEB-Q) RID, ORCIDZdroj.dok. Biologia Plantarum. - : Ústav experimentální botaniky AV ČR, v. v. i. - ISSN 0006-3134
Roč. 55, č. 3 (2011), s. 499-506Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova proteomics ; Roche MagNA Lyser Instrument ; ARABIDOPSIS-THALIANA Vědní obor RIV ED - Fyziologie CEP OC08011 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GAP501/11/1462 GA ČR - Grantová agentura ČR CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000293029800013 DOI 10.1007/s10535-011-0116-5 Anotace Mature pollen grain represents a highly desiccated structure with an extremely tough cell wall. Thanks to it, it resists common proteomic protocols. Instead, a robust homogenization has to be performed since proteins are needed to burst out of the cell to be included in the extracted proteome fraction. Here, a novel way of pollen homogenization employing Roche MagNA Lyser Instrument is presented, sparing time and laborious work. However, plant proteomics does not rely solely on perfect homogenization; also the choice of the extraction protocol is of key importance. The composition of the extraction buffer has a decisive influence on which proteome fraction will be extracted. Therefore the second part of our study is dedicated to the comparison of different extraction protocols with respect to subsequent proteomic analyses. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2012
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