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Enrichment techniques employed in phosphoproteomics
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SYSNO ASEP 0366394 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Enrichment techniques employed in phosphoproteomics Tvůrce(i) Fíla, Jan (UEB-Q) RID, ORCID
Honys, David (UEB-Q) RID, ORCIDZdroj.dok. Amino Acids. - : Springer - ISSN 0939-4451
Roč. 43, č. 3 (2012), s. 1025-1047Poč.str. 23 s. Jazyk dok. eng - angličtina Země vyd. AT - Rakousko Klíč. slova Phosphoproteomics ; Enrichment ; IMAC Vědní obor RIV ED - Fyziologie CEP GAP501/11/1462 GA ČR - Grantová agentura ČR GA522/09/0858 GA ČR - Grantová agentura ČR GA525/09/0994 GA ČR - Grantová agentura ČR OC08011 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000307536100003 DOI 10.1007/s00726-011-1111-z Anotace Rapid changes of protein phosphorylation play a crucial role in the regulation of many cellular processes. Being post-translationally modified, phosphoproteins are often present in quite low abundance and tend to co-exist with their unphosphorylated isoform within the cell. To make their identification more practicable, the use of enrichment protocols is often required. The enrichment strategies can be performed either at the level of phosphoproteins or at the level of phosphopeptides. Both approaches have their advantages and disadvantages. Most enriching strategies are based on chemical modifications, affinity chromatography to capture peptides and proteins containing negatively charged phosphate groups onto a positively charged matrix, or immunoprecipitation by phospho-specific antibodies. In this article, the most up-to-date enrichment techniques are discussed, taking into account their optimization, and highlighting their advantages and disadvantages. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2013
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