Počet záznamů: 1

Narrow-bore core–shell particles and monolithic columns in the analysis of silybin diastereoisomers

SYSNO ASEP	0366150
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Narrow-bore core–shell particles and monolithic columns in the analysis of silybin diastereoisomers
Tvůrce(i)	Marhol, Petr (MBU-M)_RID Gažák, Radek (MBU-M)_{RID, ORCID} Bednář, P. (CZ) Křen, Vladimír (MBU-M)_{RID, ORCID}
Zdroj.dok.	Journal of Separation Science. - : Wiley - ISSN 1615-9306 Roč. 34, 16-17 (2011), s. 2206-2213
Poč.str.	8 s.
Jazyk dok.	eng - angličtina
Země vyd.	DE - Německo
Klíč. slova	chromolith ; kinetex ; Core-shell
Vědní obor RIV	CC - Organická chemie
CEP	ME10027 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	GAP207/10/0288 GA ČR - Grantová agentura ČR
CEZ	AV0Z50200510 - MBU-M (2005-2011)
UT WOS	000295189400031
DOI	10.1002/jssc.201100309
Anotace	Two chromatographic narrow-bore columns, a novel 2.6 µm particle-packed KinetexTM C18 core–shell (50x2.1mm id) and monolithic Chromolith® FastGradient RP-18e (50x2mm id), were evaluated for the analysis of diastereoisomers of the flavonolignans silybin and 23-O-acetylsilybin under isocratic conditions. The main advantages of the core–shell column are markedly higher efficiency (hmin=2.8 versus 5.6 for silybin A) and better peak symmetry. The Kinetex column exhibits only a slight change in the height equivalent of the theoretical plate with a higher linear velocity of the mobile phase. The monolithic column shows notably higher selectivity in terms of selectivity factor (1.21 versus 1.12) in the analysis of critical-pair of diastereoisomers (silybin A and silybin B) and enables shorter run duration (approx. twofold) together with lower backpressure
Pracoviště	Mikrobiologický ústav
Kontakt	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Rok sběru	2012

Počet záznamů: 1