Počet záznamů: 1
First Survey of the Wheat Chromosome 5A Composition through a Next Generation Sequencing Approach
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SYSNO ASEP 0366036 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název First Survey of the Wheat Chromosome 5A Composition through a Next Generation Sequencing Approach Tvůrce(i) Vitulo, N. (IT)
Albiero, A. (IT)
Forcato, C. (IT)
Šimková, Hana (UEB-Q) RID, ORCID
Kubaláková, Marie (UEB-Q) RID
Perrotta, G. (IT)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Cattivelli, L. (IT)Celkový počet autorů 21 Zdroj.dok. PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 6, č. 10 (2011), s. 1-14Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova wheat chromosome 5A ; Chromosome arm sorting ; DNA amplification Vědní obor RIV EB - Genetika a molekulární biologie CEP ED0007/01/01 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000296186900060 DOI 10.1371/journal.pone.0026421 Anotace Wheat is one of the world’s most important crops and is characterized by a large polyploid genome. One way to reduce genome complexity is to isolate single chromosomes using flow cytometry. Low coverage DNA sequencing can provide a snapshot of individual chromosomes, allowing a fast characterization of their main features and comparison with other genomes. We used massively parallel 454 pyrosequencing to obtain a 2x coverage of wheat chromosome 5A. The resulting sequence assembly was used to identify TEs, genes and miRNAs, as well as to infer a virtual gene order based on the synteny with other grass genomes. Repetitive elements account for more than 75% of the genome. Gene content was estimated considering non-redundant reads showing at least one match to ESTs or proteins. The results indicate that the coding fraction represents 1.08% and 1.3% of the short and long arm respectively, projecting the number of genes of the whole chromosome to approximately 5,000. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2012
Počet záznamů: 1