Počet záznamů: 1

Integration of genetic and physical maps of the chickpea (Cicer arietinum L.) genome using flow-sorted chromosomes

SYSNO ASEP	0365945
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Integration of genetic and physical maps of the chickpea (Cicer arietinum L.) genome using flow-sorted chromosomes
Tvůrce(i)	Zatloukalová, Pavlína (UEB-Q) Hřibová, Eva (UEB-Q)_{RID, ORCID} Kubaláková, Marie (UEB-Q)_RID Suchánková, Pavla (UEB-Q)_RID Šimková, Hana (UEB-Q)_{RID, ORCID} Adoración, C. (ES) Kahl, G. (DE) Millán, T. (ES) Doležel, Jaroslav (UEB-Q)_{RID, ORCID}
Zdroj.dok.	Chromosome Research. - : Springer - ISSN 0967-3849 Roč. 19, č. 6 (2011), s. 729-739
Poč.str.	11 s.
Jazyk dok.	eng - angličtina
Země vyd.	NL - Nizozemsko
Klíč. slova	BAC-FISH ; Chromosome isolation ; Flow cytometric sorting
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	LC06004 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000300089200003
DOI	10.1007/s10577-011-9235-2
Anotace	Various genetic linkage maps have been established and markers linked to resistance genes been identified in cultivated chickpea. However, until now, only one linkage group (LG) has been assigned to a specific chromosome. In the present work, mitotic chromosomes were sorted using flow cytometry and used as template for PCR with primers designed for genomic regions flanking microsatellites. These primers amplify sequence-tagged microsatellite site markers. To establish a set of preferably diagnostic cytogenetic markers, the genomic distribution of various probes was verified using FISH. Moreover, a partial genomic bacterial artificial chromosome (BAC) library was constructed and putative single/low-copy BAC clones weremapped cytogenetically. As a result, two clones were identified localizing specifically to chromosomes E and H, for which no cytogenetic markers were yet available.
Pracoviště	Ústav experimentální botaniky
Kontakt	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Rok sběru	2012

Počet záznamů: 1