Počet záznamů: 1
Clonal analysis of hematopoietic progenitor cells in the zebrafish
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SYSNO ASEP 0365457 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Clonal analysis of hematopoietic progenitor cells in the zebrafish Tvůrce(i) Stachura, D.L. (US)
Svoboda, Ondřej (UMG-J)
Lau, R.P. (US)
Balla, K.M. (US)
Zon, L.I. (US)
Bartůněk, Petr (UMG-J) RID
Traver, D. (US)Zdroj.dok. Blood. - : American Society of Hematology - ISSN 0006-4971
Roč. 118, č. 5 (2011), s. 1274-1282Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova zebrafish ; in vitro culture ; hematopoiesis Vědní obor RIV EB - Genetika a molekulární biologie CEP GA310/08/0878 GA ČR - Grantová agentura ČR GAP305/10/0953 GA ČR - Grantová agentura ČR CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000293510000019 DOI 10.1182/blood-2011-01-331199 Anotace Identification of hematopoietic progenitor cells in the zebrafish has been hindered by a lack of functional assays to gauge proliferative potential and differentiation capacity. To investigate the nature of myeloerythroid progenitor cells, we developed clonal methylcellulose assays utilizing recombinant zebrafish erythropoietin (Epo) and granulocyte colony stimulating factor (Gcsf). Epo was required to support erythroid colony formation, and Gcsf to support the formation of myeloid colonies. Colonies showed distinct morphologies and were easily scored by their expression of lineage-specific fluorescent transgenes. The majority of progenitor activity was contained within the precursor light scatter fraction, and more immature precursors were present within the lymphoid fraction. These studies provide the first report of clonal hematopoietic progenitor assays in the zebrafish, and establish the number, characteristics, and kinetics of myeloerythroid progenitors during hematopoiesis. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2012
Počet záznamů: 1