Počet záznamů: 1
Interaction of late apoptotic and necrotic cells with vitronectin
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SYSNO ASEP 0365171 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Interaction of late apoptotic and necrotic cells with vitronectin Tvůrce(i) Štěpánek, Ondřej (UMG-J) RID, ORCID
Brdička, Tomáš (UMG-J) RID
Angelisová, Pavla (UMG-J) RID
Horváth, Ondřej (UMG-J)
Špička, Jiří (UMG-J)
Stockbauer, P. (CZ)
Man, Petr (MBU-M) RID, ORCID
Hořejší, Václav (UMG-J) RIDZdroj.dok. PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 6, č. 5 (2011), e19243Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova vitronectin ; apoptosis ; necrosis Vědní obor RIV EB - Genetika a molekulární biologie CEP 1M0506 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50200510 - MBU-M (2005-2011) AV0Z50520514 - UMG-J (2005-2011) UT WOS 000290224800019 DOI 10.1371/journal.pone.0019243 Anotace Vitronectin (Vn) is an abundant plasma glycoprotein identified also as a part of extracellular matrix. Vn is substantially enriched at sites of injured, fibrosing, inflamed, and tumor tissues where it is believed to be involved in wound healing and tissue remodeling. Little is known about the mechanism of Vn localization into the damaged tissues. 2E12 antibody has been described to bind a subset of late apoptotic cells. The antigen recognized by 2E12 antibody was identified as Vn. Vn binds to the late apoptotic and necrotic cells in cell cultures in vitro as well as in murine thymus and spleen in vivo. Confocal microscopy revealed that Vn binds to an intracellular cytoplasmic structure after the membrane rupture. Vitronectin could serve as a marker of membrane disruption in necrosis and apoptosis for flow cytometry analysis. Vn binding to dead cells may represent one of the mechanisms of Vn incorporation into the injured tissues. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2012
Počet záznamů: 1