Počet záznamů: 1
1,2-propanediol-trehalose mixture as a potent quantitative real-time PCR enhancer
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SYSNO ASEP 0365065 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název 1,2-propanediol-trehalose mixture as a potent quantitative real-time PCR enhancer Tvůrce(i) Horáková, Helena (UMG-J)
Polakovičová, Iva (UMG-J)
Shaik, Gouse Mohiddin (UMG-J)
Eitler, Jiří (UMG-J)
Bugajev, Viktor (UMG-J) RID
Dráberová, Lubica (UMG-J) RID
Dráber, Petr (UMG-J) RIDZdroj.dok. BMC Biotechnology
Roč. 11, - (2011), e41Poč.str. 16 s. Forma vydání http://www.biomedcentral.com/1472-6750/11/41 - http://www.biomedcentral.com/1472-6750/11/41 Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova qPCR enhancer ; trehalose ; 1,2-propanediol Vědní obor RIV EB - Genetika a molekulární biologie CEP KAN200520701 GA AV ČR - Akademie věd GD204/09/H084 GA ČR - Grantová agentura ČR GA301/09/1826 GA ČR - Grantová agentura ČR GAP302/10/1759 GA ČR - Grantová agentura ČR CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000290988500001 DOI 10.1186/1472-6750-11-41 Anotace Quantitative real-time PCR (qPCR) is becoming increasingly important for DNA genotyping and gene expression analysis. Buffers of different composition are used for various DNA dyes used in qPCR. In search for a PCR mix compatible with variety of DNA dyes, and suitable for efficient amplification of difficult-to-amplify DNA templates, such as those in whole blood, of medium size and/or GC-rich, we found excellent performance of a PCR mix supplemented with 1 M 1,2-propanediol and 0.2 M trehalose (PT enhancer). These two additives together decreased DNA melting temperature and efficiently neutralized PCR inhibitors present in blood samples. They also made possible more efficient amplification of GC-rich templates than betaine and other previously described additives. The data indicate that PCR mixes supplemented with PT enhancer are suitable for DNA amplification in the presence of various DNA dyes and for a variety of templates which otherwise can be amplified with difficulty. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2012
Počet záznamů: 1