Počet záznamů: 1
Frequency of aneuploidy related to age in porcine oocytes
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SYSNO ASEP 0365035 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Frequency of aneuploidy related to age in porcine oocytes Tvůrce(i) Horňák, M. (CZ)
Jeseta, M. (CZ)
Musilová, P. (CZ)
Pavlok, Antonín (UZFG-Y) RID
Kubelka, Michal (UZFG-Y) RID, ORCID
Motlík, Jan (UZFG-Y) RID, ORCID
Rubeš, J. (CZ)
Anger, Martin (UZFG-Y) ORCIDCelkový počet autorů 8 Zdroj.dok. PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 6, č. 4 (2011), s. 1-5Poč.str. 5 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova porcine ; oocytes ; aneuploidy Vědní obor RIV EE - Mikrobiologie, virologie CEP GA523/09/0743 GA ČR - Grantová agentura ČR IAA501620801 GA AV ČR - Akademie věd CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000290019400013 DOI 10.1371/journal.pone.0018892 Anotace It is generally accepted that mammalian oocytes are frequently suffering from chromosome segregation errors during meiosis I, which have severe consequences, including pregnancy loss, developmental disorders and mental retardation. In a search for physiologically more relevant model than rodent oocytes to study this phenomenon, we have employed comparative genomic hybridization (CGH), combined with whole genome amplification (WGA), to study the frequency of aneuploidy in porcine oocytes, including rare cells obtained from aged animals. Using this method, we were able to analyze segregation pattern of each individual chromosome during meiosis I. In contrast to the previous reports where conventional methods, such as chromosome spreads or FISH, were used to estimate frequency of aneuploidy, our results presented here show, that the frequency of this phenomenon was overestimated in porcine oocytes. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2012
Počet záznamů: 1