Počet záznamů: 1

Transcriptomic analysis of in vivo and in vitro produced bovine embryos revealed a developmental change in cullin 1 expression during maternal-to-embryonic transition

SYSNO ASEP	0364524
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Transcriptomic analysis of in vivo and in vitro produced bovine embryos revealed a developmental change in cullin 1 expression during maternal-to-embryonic transition
Tvůrce(i)	Vodičková Kepková, Kateřina (UZFG-Y)_ORCID Vodička, Petr (UZFG-Y)_ORCID Toralová, Tereza (UZFG-Y)_ORCID Lopatářová, M. (CZ) Čech, S. (CZ) Doležel, R. (CZ) Havlíček, V. (CZ) Besenfelder, U. (AT) Kuzmany, H. (AT) Sirard, M. A. (CA) Laurinčik, J. (SK) Kaňka, Jiří (UZFG-Y)_{RID, ORCID}
Celkový počet autorů	12
Zdroj.dok.	Theriogenelogy. - : Elsevier - ISSN 0093-691X Roč. 75, č. 9 (2011), s. 1582-1595
Poč.str.	14 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	OOCYTE MATURATION MEDIA ; MESSENGER-RNA LEVELS ; EARLY EMBRYOGENESIS
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	1M0538 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	GA523/09/1035 GA ČR - Grantová agentura ČR
	GD204/09/H084 GA ČR - Grantová agentura ČR
CEZ	AV0Z50450515 - UZFG-Y (2005-2011)
UT WOS	000290925500002
DOI	10.1016/j.theriogenology.2010.12.019
Anotace	Pre-implantation embryos derived by in vitro fertilization differ in their developmental potential from embryos obtained in vivo. In order to characterize changes in gene expression profiles caused by in vitro culture environment, we employed microarray constructed from bovine oocyte and preimplantation embryo-specific cDNAs (BlueChip, Universite Laval, Quebec). The analysis revealed changes in the level of 134 transcripts between in vitro derived (cultured in COOK BVC/BVB media) and in vivo derived 4-cell stage embryos and 97 transcripts were differentially expressed between 8-cell stage in vitro and in vivo embryos. The expression profiles of 7 selected transcripts (BUB3, CUL1, FBL, NOLC1, PCAF, GABPA and CNOT4) were studied in detail. We have identified a switch from Cullin 1-like transcript variant 1 to Cullin 1 transcript variant 3 (UniGene IDs BT.36789 and BT.6490, respectively) expressions around the time of bovine major gene activation (8-cell stage).
Pracoviště	Ústav živočišné fyziologie a genetiky
Kontakt	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Rok sběru	2012

Počet záznamů: 1