Počet záznamů: 1
Evidence for Integrity of Parental Genomes in the Diploid Hybridogenetic Water Frog Pelophylax esculentus by Genomic in situ Hybridization
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SYSNO ASEP 0364474 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Evidence for Integrity of Parental Genomes in the Diploid Hybridogenetic Water Frog Pelophylax esculentus by Genomic in situ Hybridization Tvůrce(i) Zalésna, A. (PL)
Choleva, Lukáš (UZFG-Y) RID, ORCID
Ogielska, M. (PL)
Rábová, Marie (UZFG-Y) RID
Marec, František (BC-A) RID, ORCID
Ráb, Petr (UZFG-Y) RID, ORCIDCelkový počet autorů 6 Zdroj.dok. Cytogenetic and Genome Research. - : Karger - ISSN 1424-8581
Roč. 134, č. 3 (2011), s. 206-212Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova Amphibia ; Chromosomes ; Genomic in situ hybridization (GISH) Vědní obor RIV EB - Genetika a molekulární biologie CEP LC06073 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA523/09/2106 GA ČR - Grantová agentura ČR CEZ AV0Z50450515 - UZFG-Y (2005-2011) AV0Z50070508 - ENTU-I, BC-A (2005-2011) UT WOS 000292469000006 DOI 10.1159/000327716 Anotace The Western Palearctic water frogs Pelophylax ridibundus and P. lessonae were identified as parental (sexual) species and P. esculentus as their interspecific, hybridogenetically reproducing hybrid with hemiclonal heredity. We used genomic in situ hybridization (GISH) to identify parental chromosomes of P. lessonae and P. ridibundus in diploid P. esculentus karyotypes (2n = 26). GISH probes were made by fluorochrome labeling of total genomic DNA extracted from the sexual progenitors. The labeled probe from one species was hybridized to chromosomes of P. esculentus in the presence of excess of unlabeled genomic DNA from the other species. Thus, the P. lessonae probe was blocked by P. ridibundus unlabeled DNA, and vice versa. We successfully discriminated each of the 13 respective parental chromosomes in metaphase complements of the hybrids according to species-specific hybridization signals. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2012
Počet záznamů: 1