Počet záznamů: 1
Single-cell gene-expression profiling and its potential diagnostic applications
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SYSNO ASEP 0364328 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Single-cell gene-expression profiling and its potential diagnostic applications Tvůrce(i) Stahlberg, A. (SE)
Kubista, Mikael (BTO-N) RID
Aman, P. (SE)Zdroj.dok. Expert Review of Molecular Diagnostics . - : Taylor & Francis - ISSN 1473-7159
Roč. 11, č. 7 (2011), s. 735-740Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova gene-expression profiling ; RT-qPCR ; single-cell gene-expression profiling Vědní obor RIV EB - Genetika a molekulární biologie CEP GAP303/10/1338 GA ČR - Grantová agentura ČR GA301/09/1752 GA ČR - Grantová agentura ČR CEZ AV0Z50520701 - BTO-N (2007-2013) UT WOS 000294848400014 DOI 10.1586/ERM.11.60 Anotace Gene-expression profiling has been successfully applied in various diagnostic applications, but its full capacity is yet to be realized. Samples are generally prepared from a mixture of different cells that are present in unknown proportions. Cells are, in many aspects, unique in their characteristics and this heterogeneity confounds the expression profile. The development of new and robust techniques to measure gene expression in single cells opens new avenues in molecular medicine. Today, gene-expression profiles of individual cells can be measured with high precision and accuracy, identifying different cell types as well as revealing heterogeneity among cells of the same kind. Here, we review practical aspects of single-cell gene-expression profiling using reverse transcription quantitative real-time PCR and its potential use in diagnostics Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2012
Počet záznamů: 1