Počet záznamů: 1

Expression of Human papillomavirus 16 E7ggg oncoprotein on N- and C-terminus of Potato virus X coat protein in bacterial and plant cells

SYSNO ASEP	0364095
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Expression of Human papillomavirus 16 E7ggg oncoprotein on N- and C-terminus of Potato virus X coat protein in bacterial and plant cells
Tvůrce(i)	Plchová, Helena (UEB-Q)_{RID, ORCID} Moravec, Tomáš (UEB-Q)_{RID, ORCID} Hoffmeisterová, Hana (UEB-Q)_RID Folwarczna, Jitka (UEB-Q) Čeřovská, Noemi (UEB-Q)_{RID, ORCID}
Zdroj.dok.	Protein Expression and Purification. - : Elsevier - ISSN 1046-5928 Roč. 77, č. 2 (2011), s. 146-152
Poč.str.	7 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	Bacterial expression ; Human papillomavirus ; Oncoprotein E7
Vědní obor RIV	EI - Biotechnologie a bionika
CEP	GA521/09/1525 GA ČR - Grantová agentura ČR
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000288591900003
DOI	10.1016/j.pep.2011.01.008
Anotace	The E7 oncoprotein from Human papillomavirus type 16 (HPV16) is an attractive candidate for anti-cancer therapeutical vaccine development. In this study, we engineered different fusions of mutagenized coding sequence of E7 oncoprotein (E7ggg) with coat protein of Potato virus X (PVX CP) both on 5`- and 3`-terminus of PVX CP and evaluated the influence of the length of linker (no linker, 4, 15 aa) connecting PVX CP and E7ggg on their production. At first the expression in Escherichia coil was conducted to assess the characteristics of the recombinant protein prior to be further produced in plants, that is, resultant proteins were used for screening of their immunological reactivity with antibodies against PVX CP and E7. Fusion proteins successfully expressed in bacteria and plants were partially purified and their reactivity and ability to form virus-like particles were evaluated with anti-E7 antibodies.
Pracoviště	Ústav experimentální botaniky
Kontakt	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Rok sběru	2012

Počet záznamů: 1