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E-selectin is a viable route of infection for polymer-coated adenovirus retargeting in TNF-.alpha.-activated human umbilical vein endothelial cells
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SYSNO ASEP 0362894 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název E-selectin is a viable route of infection for polymer-coated adenovirus retargeting in TNF-.alpha.-activated human umbilical vein endothelial cells Tvůrce(i) Bachtarzi, H. (GB)
Stevenson, M. (GB)
Šubr, Vladimír (UMCH-V) RID, ORCID
Seymour, L. W. (GB)
Fisher, K. D. (GB)Zdroj.dok. Journal of Drug Targeting - ISSN 1061-186X
Roč. 19, č. 8 (2011), s. 690-700Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova polymer-coated virus ; vascular targeting ; inflammation Vědní obor RIV CD - Makromolekulární chemie CEZ AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000293743800012 DOI 10.3109/1061186X.2010.547585 Anotace Retargeting of adenovirus via E-selectin as a viable pathway of infection in tumor necrosis factor-α (TNF-α)-activated human umbilical vein endothelial cells (HUVECs) was investigated. E1, E3-deleted Ad5 expressing cytomegalovirus immediate-early promoter-driven luciferase (Adluc) was coated with a reactive multivalent copolymer based on poly [N-(2-hydroxypropyl) methacrylamide] to generate pHPMA-adenovirus (pcAdluc). This was then retargeted by covalent attachment of a mouse antihuman E-selectin monoclonal antibody (MHES mAb). MHESpcAdluc was efficiently taken up into HUVECs, generating a high level of transduction in TNF-α-treated E-selectin positive cells but not in untreated receptor-negative cells. Our results suggest that E-selectin could be a valuable target for gene transfer strategies internalizing polymer-coated adenovirus particles through a viable receptor-mediated endocytosis pathway, generating adequate levels of transgene expression per virus genome copy. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2012
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