Počet záznamů: 1
Metallochelating liposomes with associated lipophilised norAbuMDP as biocompatible platform for construction of vaccines with recombinant His-tagged 3 antigens: Preparation, structural study and immune response towards rHsp90
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SYSNO ASEP 0361499 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Metallochelating liposomes with associated lipophilised norAbuMDP as biocompatible platform for construction of vaccines with recombinant His-tagged 3 antigens: Preparation, structural study and immune response towards rHsp90 Tvůrce(i) Mašek, J. (CZ)
Bartheldyová, E. (CZ)
Turánek-Knotigová, P. (CZ)
Škrabalová, M. (CZ)
Korvasová, Z. (CZ)
Plocková, J. (CZ)
Koudelka, Š. (CZ)
Škodová, P. (CZ)
Kulich, P. (CZ)
Křupka, M. (CZ)
Zachová, K. (CZ)
Czerneková, L. (CZ)
Horynová, M. (CZ)
Kratochvílová, Irena (FZU-D) RID, ORCID, SAI
Miller, A. D. (GB)
Zyka, Daniel (UOCHB-X) RID
Michálek, J. (CZ)
Vrbová, J. (CZ)
Šebela, M. (CZ)
Ledvina, Miroslav (UOCHB-X) RID
Raška, M. (CZ)
Turánek, J. (CZ)Zdroj.dok. Journal of Controlled Release. - : Elsevier - ISSN 0168-3659
Roč. 151, č. 2 (2011), s. 193-201Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova recombinant vaccine ; liposome Vědní obor RIV EC - Imunologie CEP GAP304/10/1951 GA ČR - Grantová agentura ČR KAN200520703 GA AV ČR - Akademie věd KAN200100801 GA AV ČR - Akademie věd CEZ AV0Z10100520 - FZU-D (2005-2011) AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000291845600012 DOI 10.1016/j.jconrel.2011.01.016 Anotace Hsp90-CA is present in cell wall of Candida pseudohyphae or hyphae - typical pathogenic morphotype for 31 both systemic and mucosal Candida infections. Heat shock protein from Candida albicans (hsp90-CA) is an 32 important target for protective antibodies during disseminated candidiasis of experimental mice and human. 33 His-tagged protein rHsp90 was prepared and used as the antigen for preparation of experimental 34 recombinant liposomal vaccine. Nickel-chelating liposomes (the size around 100 nm, PDI≤0.1) were 35 prepared from the mixture of egg phosphatidyl choline and nickel-chelating lipid DOGS-NTA-Ni (molar ratio 36 95:5%) by hydration of lipid film and extrusion methods. New non-pyrogenic hydrophobised derivative of 37 MDP (C18-O-6-norAbuMDP) was incorporated into liposomes as adjuvans. by metallochelating bond and the structure of these proteoliposomes 39 was studied by dynamic light scattering, AF microscopy, TEM and GPC. Pracoviště Fyzikální ústav Kontakt Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579 Rok sběru 2012
Počet záznamů: 1