Počet záznamů: 1
High-yield continuous production of nicotinic acid via nitrile hydratase–amidase cascade reactions using cascade CSMRs
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SYSNO ASEP 0360788 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název High-yield continuous production of nicotinic acid via nitrile hydratase–amidase cascade reactions using cascade CSMRs Tvůrce(i) Cantarella, L. (IT)
Gallifuoco, A. (IT)
Malandra, A. (IT)
Martínková, Ludmila (MBU-M) RID, ORCID
Spera, A. (IT)
Cantarella, M. (IT)Zdroj.dok. Enzyme and Microbial Technology. - : Elsevier - ISSN 0141-0229
Roč. 48, 4-5 (2011), 345-350Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Nitrile hydratase-amidase cascade system ; 3-Cyanopyridine bioconversion ; Nicotinic acid Vědní obor RIV EE - Mikrobiologie, virologie CEP OC09046 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000289930900007 DOI 10.1016/j.enzmictec.2010.12.010 Anotace High yields of nicotinic acid from 3-cyanopyridine bioconversion were obtained by exploiting the in situ nitrile hydratase–amidase enzymatic cascade system of Microbacterium imperiale CBS 498-74. Experiments were carried out in continuously stirred tank UF-membrane bioreactors (CSMRs) arranged in series. This reactor configuration enables both enzymes, involved in the cascade reaction, to work with optimized kinetics, without any purification, exploiting their differing temperature dependences. To this end, the first CSMR, optimized for the properties of the NHase, was operated (i) at low temperature (5◦C), limiting inactivation of the more fragile enzyme, nitrile hydratase, (ii) with a high residence time (24h) to overcome reaction rate limitation Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2012
Počet záznamů: 1