Počet záznamů: 1
Biotransformation of nitriles to hydroxamic acids via a nitrile hydratase–amidase cascade reaction
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SYSNO ASEP 0360786 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Biotransformation of nitriles to hydroxamic acids via a nitrile hydratase–amidase cascade reaction Tvůrce(i) Vejvoda, Vojtěch (MBU-M)
Martínková, Ludmila (MBU-M) RID, ORCID
Veselá, Alicja Barbara (MBU-M) RID
Kaplan, Ondřej (MBU-M)
Lutz-Wahl, S. (DE)
Fischer, L. (DE)
Uhnáková, Bronislava (MBU-M)Zdroj.dok. Journal of Molecular Catalysis B-Enzymatic. - : Elsevier - ISSN 1381-1177
Roč. 71, 1-2 (2011), s. 51-55Poč.str. 5 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Nitrile hydratase ; Rhodococcus erythropolis ; Amidase Vědní obor RIV EE - Mikrobiologie, virologie CEP LC06010 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy OC09046 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000291451000008 DOI 10.1016/j.molcatb.2011.03.008 Anotace The transformation of benzonitrile into benzohydroxamic acid was performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step was catalyzed by either a cell-free extract from Rhodococcus erythropolis A4 (nitrile hydratase) or cell-free extracts from recombinant Escherichia coli strains expressing nitrile hydratases from Raoultella terrigena 77.1 and Klebsiella oxytoca 38.1.2; the biocatalyst in the second step was a cellfree extract from R. erythropolis A4 (amidase). When using the cell-free extract from R. erythropolis A4 in the first step, the hydrolytic amidase activity was suppressed by ammonium ions, which, however, did not inhibit the acyl transfer reaction catalyzed by the same enzyme. Aromatic and aliphatic nitriles were examined as substrates of the recombinant nitrile hydratases by using a colorimetric assay of hydroxamic acids produced in a coupled acyl transfer reaction Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2012
Počet záznamů: 1