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Pentapeptide-modified poly(N,N-diethylacrylamide) hydrogel scaffolds for tissue engineering

SYSNO ASEP	0360755
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Pentapeptide-modified poly(N,N-diethylacrylamide) hydrogel scaffolds for tissue engineering
Tvůrce(i)	Horák, Daniel (UMCH-V)_{RID, ORCID} Matulka, K. (CZ) Hlídková, Helena (UMCH-V)_{RID, ORCID} Lapčíková, Monika (UMCH-V)_RID Beneš, Milan J. (UMCH-V)_RID Jaroš, J. (CZ) Hampl, Aleš (UEM-P) Dvořák, Petr (UEM-P)
Zdroj.dok.	Journal of Biomedical Materials Research. Part B. - : Wiley - ISSN 1552-4973 98B, č. 1 (2011), s. 54-67
Poč.str.	14 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	poly(N,N-diethylacrylamide) ; porosity ; YIGSR peptide
Vědní obor RIV	FH - Neurologie, neurochirurgie, neurovědy
CEP	1M0538 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	GAP304/11/0731 GA ČR - Grantová agentura ČR
CEZ	AV0Z40500505 - UMCH-V (2005-2011)
	AV0Z50390512 - UEM-P (2005-2011)
	AV0Z50390703 - UEM-P (2007-2013)
UT WOS	000291598900007
DOI	10.1002/jbm.b.31832
Anotace	Poly(N,N-diethylacrylamide) (PDEAAm) hydrogel scaffolds were prepared by radical copolymerization of N,Ndiethylacrylamide (DEAAm), N,N0-methylenebisacrylamide and methacrylic acid in the presence of (NH4)2SO4 or NaCl. The hydrogels were characterized by low-vacuum scanning electron microscopy in the water-swollen state, water and cyclohexane regain, and by mercury porosimetry. The pentapeptide, YIGSR-NH2, was immobilized on the hydrogel. Human embryonic stem cells (hESCs) were cultured with the hydrogels to test their biocompatibility. The results suggest that the PDEAAm hydrogel scaffolds are nontoxic and support hESC attachment and proliferation, and that interconnected pores of the scaffolds are important for hESC cultivation. Immobilization of YIGSR-NH2 pentapeptide on the PDEAAm surface improved both adhesion and growth of hESCs compared with the unmodified hydrogel. The YIGSRNH2- modified PDEAAm hydrogels may be a useful tool for tissue-engineering purposes.
Pracoviště	Ústav makromolekulární chemie
Kontakt	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Rok sběru	2012

Počet záznamů: 1