Počet záznamů: 1
Genome mining for the discovery of new nitrilases in filamentous fungi
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SYSNO ASEP 0360696 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Genome mining for the discovery of new nitrilases in filamentous fungi Tvůrce(i) Kaplan, Ondřej (MBU-M)
Bezouška, Karel (MBU-M)
Malandra, Anna (MBU-M) ORCID
Veselá, Alicja Barbara (MBU-M) RID
Petříčková, Alena (MBU-M) RID
Felsberg, Jürgen (MBU-M)
Rinágelová, Anna (MBU-M)
Křen, Vladimír (MBU-M) RID, ORCID
Martínková, Ludmila (MBU-M) RID, ORCIDZdroj.dok. Biotechnology Letters. - : Springer - ISSN 0141-5492
Roč. 33, č. 2 (2011), 309-312Poč.str. 4 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Aromatic nitrilase ; Arylacetonitrilase ; Aspergillus niger Vědní obor RIV EE - Mikrobiologie, virologie CEP IAA500200708 GA AV ČR - Akademie věd LC06010 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy OC09046 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy FT-TA5/043 GA MPO - Ministerstvo průmyslu a obchodu GD305/09/H008 GA ČR - Grantová agentura ČR CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000286198700014 DOI 10.1007/s10529-010-0421-7 Anotace Our aim is to describe new fungal nitrilases whose sequences were published but whose catalytic properties were unknown. We adapted for expression in E. coli three of the genes and confirmed that the enzymes acted on organic nitriles. The genome mining approach was used to search for nitrilases in filamentous fungi. Synthetic genes encoding nitrilases in Aspergillus niger, Gibberella moniliformis and Neurospora crassa were expressed in Escherichia coli. This is the first heterologous expression of fungal enzymes of this type. The recombinant enzyme derived from G. moniliformis was an aromatic nitrilase with an aktivity of 390 U l culture with benzonitrile as substrate. This was much less than the activities of the recombinant enzymes derived from A. niger and N. crassa that had activities of 2500 and 2700 U l culture, respectively, with phenylacetonitrile as substrate Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2012
Počet záznamů: 1