Počet záznamů: 1

A comprehensive study in triblock copolymer membrane interaction

SYSNO ASEP	0360337
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	A comprehensive study in triblock copolymer membrane interaction
Tvůrce(i)	Pembouong, G. (FR) Morellet, N. (FR) Kral, Teresa (UFCH-W)_{RID, ORCID} Hof, Martin (UFCH-W)_{RID, ORCID} Scherman, D. (FR) Bureau, M.-F. (FR) Mignet, N. (FR)
Zdroj.dok.	Journal of Controlled Release. - : Elsevier - ISSN 0168-3659 Roč. 151, č. 1 (2011), s. 57-64
Poč.str.	8 s.
Jazyk dok.	eng - angličtina
Země vyd.	NL - Nizozemsko
Klíč. slova	poloxamer ; pluronic ; liposomes
Vědní obor RIV	CF - Fyzikální chemie a teoretická chemie
CEP	IAA400400621 GA AV ČR - Akademie věd
	LC06063 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
CEZ	AV0Z40400503 - UFCH-W (2005-2011)
UT WOS	000291240900009
DOI	10.1016/j.jconrel.2011.01.007
Anotace	Poloxamers are triblock copolymers made of poly(ethylene glycol)-(poly(propylene glycol))-poly(ethylene glycol). They have been shown to enhance gene transfer in the muscle, and co-administration of polymers and DNA appeared to be crucial to obtain this effect. It is questionable then if some interaction occurs between polymers and DNA. Polymer interaction with membranes represents a second crucial point due to the central hydrophobic part of the triblock copolymers. Besides, the question of the polymer spanning or adsorbing to the surface has not been solved by now. We addressed these issues by means of sensitive techniques that allowed working in diluted conditions and gaining in comprehension of gene transfection. By means of simultaneous time-correlated single-photon counting and fluorescence correlation spectroscopy, we have shown that the diffusion time of a single DNA molecule and PicoGreen lifetime was not altered in the presence of the triblock copolymer L64.
Pracoviště	Ústav fyzikální chemie J.Heyrovského
Kontakt	Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
Rok sběru	2012

Počet záznamů: 1