Počet záznamů: 1

Functional characterization of two defensin isoforms of the hard tick Ixodes ricinus

SYSNO ASEP	0360134
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Functional characterization of two defensin isoforms of the hard tick Ixodes ricinus
Tvůrce(i)	Chrudimská, Tereza (BC-A) Slaninová, Jiřina (UOCHB-X) Rudenko, Natalia (BC-A)_{RID, ORCID} Růžek, Daniel (BC-A)_{RID, ORCID} Grubhoffer, Libor (BC-A)_{RID, ORCID}
Zdroj.dok.	Parasites & Vectors. - : BioMed Central - ISSN 1756-3305 Roč. 4, č. 1 (2011), e63
Poč.str.	9 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	defensin ; antimicrobial compounds ; Ixodes ricinus
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	LC06009 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	GA206/09/1782 GA ČR - Grantová agentura ČR
	GD206/09/H026 GA ČR - Grantová agentura ČR
	GAP302/11/1901 GA ČR - Grantová agentura ČR
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
	AV0Z40550506 - UOCHB-X (2005-2011)
UT WOS	000291094400001
DOI	10.1186/1756-3305-4-63
Anotace	The immune system of ticks is stimulated to produce many pharmacologically active molecules during feeding and especially during pathogen invasion. Defensins represent a specific group of antimicrobial compounds with 6 conserved cysteine residues. Two isoforms of the defensin gene (def1 and def2) were identified in the European tick Ixodes ricinus. Expression of both genes was induced in different tick organs by a blood feeding or pathogen injection. Synthetic peptides def1 and def2 were shown to have antimicrobial activitiy against Gram+ bacteria, while Gram- bacteria, yeast, Tick Borne Encephalitis and West Nile Viruses were shown to be insensitive. In addition to antimicrobial activities, the hemolysis effect of def1 and def2 on human erythrocytes was also established. Defensins may play an important role in defence against different pathogens. Moreover this is a first report of a one amino acid substitution in a defensins molecule and its impact on antimicrobial activity.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2012

Počet záznamů: 1