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Bioactive support for cell cultivation and potential grafting. Part1. Surface modification of 2-hydroxyethyl methacrylate hydrogels for avidin immobilization
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SYSNO ASEP 0359861 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Bioactive support for cell cultivation and potential grafting. Part1. Surface modification of 2-hydroxyethyl methacrylate hydrogels for avidin immobilization Tvůrce(i) Hobzová, Radka (UMCH-V) RID, ORCID
Přádný, Martin (UMCH-V) RID
Zhunusbekova, Nazym M. (UMCH-V)
Širc, Jakub (UMCH-V) RID, ORCID
Guryča, Vilém (UMCH-V)
Michálek, Jiří (UMCH-V) RID, ORCIDZdroj.dok. E-Polymers. - : EUROPEAN POLYMER FEDERATION - ISSN 1618-7229
-, 043 (2011), s. 1-17Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova hydrogel ; poly(2-hydroxyethyl methacrylate) ; surface modification Vědní obor RIV CD - Makromolekulární chemie CEP 1QS400500558 GA AV ČR - Akademie věd CEZ AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000290474600001 DOI 10.1515/epoly.2011.11.1.474 Anotace Synthetic hydrogels are often used in biomedical applications as many of them are compatible with living tissue and moreover they meet the most criteria for artificial tissue properties. To enhance the cell adhesion and proliferation, it is desirable to modify the polymer surface by some bioactive compounds (e.g. saccharides, proteins). In this work, firstly carboxyl-enriched hydrogels were prepared by various methods: by copolymerization of 2 hydroxyethyl methacrylate with methacrylic acid, by hydrolysis or oxidation of poly(2 hydroxyethyl methacrylate). Subsequently, avidin was immobilized to the modified hydrogel surfaces either electrostatically through dissociated carboxylic groups or covalently through carboxylic groups activated by N-hydroxysuccinimide. Preliminary biological tests showed that hydrogels with immobilized avidin provides better adhesion and proliferation of keratinocytes compared to supports without avidin. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2012 Elektronická adresa http://www.e-polymers.org/journal/abstract.cfm?abstract_Id=3616
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