Počet záznamů: 1
Serum/Plasma Proteomics Metods and Protocols
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SYSNO ASEP 0359480 Druh ASEP M - Kapitola v monografii Zařazení RIV C - Kapitola v knize Název Intact protein separation by 1 and 2dimension liquid chromatography for the comparative proteomic separation of partitioned serum or plasma Tvůrce(i) Sheng, S. (US)
Skalníková, Helena (UZFG-Y) RID, ORCID
Meng, A. (US)
Fu, Q. (CN)
Everett, A. (US)
Van Eyk, J. (US)Zdroj.dok. Serum/Plasma Proteomics Metods and Protocols. - NEW YORK : Humana Press, 2011 - ISBN 978-1-61779-067-6 Rozsah stran s. 29-46 Poč.str. 17 s. Poč.výt. 300 Poč.str.knihy 397 Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova liquid chromatography ; chromatofocusing ; reversed phase Vědní obor RIV EB - Genetika a molekulární biologie CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000289957500002 Anotace Serum and plasma-based biomarker discovery requires technologies with specific capabilities: sufficient proteome coverage and depth; technical reproducibly; and the scalability to enable analysis on substantial volumes of a large number of samples at reasonable cost. We have shown that plasma samples processed using ProteomeLab™ IgY-12 LC10 Proteome Partitioning Kits to selectively remove the 12 most highly-abundant proteins, followed by intact protein separation by two-dimensional liquid chromatography (2DLC, chromatofocusing and reversed phase) can unique enrich for middle –lower-abundant proteins. Equally, 1DLC (reversed phase) separation of intact proteins is complementary to 2DLC. The serial use of a single piece of equipment can be prohibitively time-consuming and thus, this chapter also describes the harmonization of multiple LC instruments in order to minimize technical variation and ensure reproducibility. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2012
Počet záznamů: 1