Počet záznamů: 1

Phenyl- and benzylurea cytokinins as competitive inhibitors of cytokinin oxidase/dehydrogenase: a structural study

SYSNO ASEP	0359309
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Phenyl- and benzylurea cytokinins as competitive inhibitors of cytokinin oxidase/dehydrogenase: a structural study
Tvůrce(i)	Kopečný, D. (CZ) Briozzo, P. (FR) Popelková, H. (US) Šebela, M. (CZ) Končitíková, R. (CZ) Spíchal, Lukáš (UEB-Q)_{RID, ORCID} Nisler, Jaroslav (UEB-Q)_{RID, ORCID} Madzak, C. (FR) Frébort, Ivo (UEB-Q) Laloue, M. (FR) Houba-Herin, N. (FR)
Celkový počet autorů	11
Zdroj.dok.	Biochimie. - : Elsevier - ISSN 0300-9084 Roč. 92, č. 8 (2010), s. 1052-1062
Poč.str.	11 s.
Jazyk dok.	eng - angličtina
Země vyd.	FR - Francie
Klíč. slova	benzylurea ; crystal structure ; cytokinin oxidase/dehydrogenase
Vědní obor RIV	CE - Biochemie
CEP	GA522/08/0555 GA ČR - Grantová agentura ČR
	GA301/08/1649 GA ČR - Grantová agentura ČR
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000280570800018
DOI	10.1016/j.biochi.2010.05.006
Anotace	Cytokinin oxidase/dehydrogenase (CKO) is a flavoenzyme, which irreversibly degrades the plant hormones cytokinins. An inhibitory study with numerous urea derivatives was undertaken using the maize enzyme (ZmCKO1) and the crystal structure of ZmCKO1 in a complex with N-(2-chloro-pyridin-4-yl)-N´-phenylurea (CPPU) was solved. Subsequently, site-directed mutagenesis of L492 and E381 residues involved in the inhibitor binding was performed. The crystal structures of L492A mutant in a complex with CPPU and N-(2-chloro-pyridin-4-yl)-N´-benzylurea (CPBU) were solved and confirm the importance of a stacking interaction between the 2-chloro-4-pyridinyl ring of the inhibitor and the isoalloxazine ring of the FAD cofactor. As highly specific CKO inhibitors without undesired side effects are of major interest for physiological studies, all studied compounds were further analyzed for cytokinin activity in the Amaranthus bioassay and for binding to the Arabidopsis cytokinin receptors AHK3 and AHK4.
Pracoviště	Ústav experimentální botaniky
Kontakt	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Rok sběru	2012

Počet záznamů: 1