Počet záznamů: 1
Analysis of 2-methylthio-derivatives of isoprenoid cytokinins by liquid chromatography-tandem mass spectrometry
- 1.
SYSNO ASEP 0359308 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Analysis of 2-methylthio-derivatives of isoprenoid cytokinins by liquid chromatography-tandem mass spectrometry Tvůrce(i) Tarkowski, Petr (UEB-Q)
Václavíková, Kateřina (UEB-Q)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Pertry, I. (BE)
Hanuš, Jan (UEB-Q)
Whenham, R. (GB)
Vereecke, D. (BE)
Šebela, M. (CZ)
Strnad, Miroslav (UEB-Q) RID, ORCIDCelkový počet autorů 9 Zdroj.dok. Analytica Chimica Acta. - : Elsevier - ISSN 0003-2670
Roč. 680, 1-2 (2010), s. 86-91Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Cytokinins ; Rhodococcus fascians ; High-performance liquid chromatography Vědní obor RIV CE - Biochemie CEP GA301/08/1649 GA ČR - Grantová agentura ČR CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000284385800013 DOI 10.1016/j.aca.2010.09.020 Anotace A sensitive and reliable high-performance liquid chromatographic method with tandem mass spectrometric detection has been developed and used for the determination of 2-methylthio-cytokinin derivatives produced by the phytopathogenic actinomycete Rhodococcus fascians. The cultivation medium containing secreted cytokinins was concentrated and subjected to a solid-phase extraction (C18 and ion-exchange). The purified samples were further separated and analyzed by HPLC-ESI-MS/MS. This allowed to achieve chromatographic resolution of six highly hydrophobic cytokinin species including 2-methylthio-isopentenyladenine, 2-methylthio-isopentenyladenosine, 2-methylthio-trans-zeatin and 2-methylthio-trans-zeatin riboside and their cis-isomers. Quantification was performed by a standard isotope dilution method using a multiple-reaction monitoring (MRM) mode. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2011
Počet záznamů: 1