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An improved in vivo deuterium labeling method for measuring the biosynthetic rate of cytokinins

SYSNO ASEP	0359292
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	An improved in vivo deuterium labeling method for measuring the biosynthetic rate of cytokinins
Tvůrce(i)	Tarkowski, Petr (UEB-Q) Floková, K. (CZ) Václavíková, Kateřina (UEB-Q) Jaworek, P. (CZ) Raus, M. (CZ) Nordström, A. (SE) Novák, Ondřej (UEB-Q)_{RID, ORCID, SAI} Doležal, Karel (UEB-Q)_{RID, ORCID} Šebela, M. (CZ) Frébortová, Jitka (UEB-Q)_{RID, ORCID}
Celkový počet autorů	10
Zdroj.dok.	Molecules. - : MDPI Roč. 15, č. 12 (2010), s. 9214-9229
Poč.str.	16 s.
Jazyk dok.	eng - angličtina
Země vyd.	CH - Švýcarsko
Klíč. slova	cytokinin ; deuterium labelling ; biosynthetic rate
Vědní obor RIV	CE - Biochemie
CEP	GA522/08/0920 GA ČR - Grantová agentura ČR
	ED0017/01/01 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	GA301/08/1649 GA ČR - Grantová agentura ČR
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000285709000046
DOI	10.3390/molecules15129214
Anotace	A set of 11 relevant isoprenoid cytokinins, including zeatin isomers, was separated by ultra performance liquid chromatography in less than 6 min. The iP-type cytokinins were observed to give rise to a previously-unknown fragment at m/z 69; we suggest that the diagnostic (204-69) transition can be used to monitor the biosynthetic rate of isopentenyladenine. Furthermore, we found that by treating the cytokinin nucleotides with alkaline phosphatase prior to analysis, the sensitivity of the detection process could be increased. In addition, derivatization (propionylation) improved the ESIMS response by increasing the analytes' hydrophobicity. Indeed, the ESI-MS response of propionylated isopentenyladenosine was about 34% higher than that of its underivatized counterpart. Moreover, the response of the derivatized zeatin ribosides was about 75% higher than that of underivatized zeatin ribosides. Finally, we created a web-based calculator (IZOTOP) that facilitates MS/MS data processing.
Pracoviště	Ústav experimentální botaniky
Kontakt	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Rok sběru	2011

Počet záznamů: 1