Počet záznamů: 1

Molecular characterization of Cryptosporidium spp. in pre-weaned dairy calves in the Czech Republic: Absence of C. ryanae and management-associated distribution of C. andersoni, C. bovis and C. parvum subtypes

SYSNO ASEP	0359225
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Molecular characterization of Cryptosporidium spp. in pre-weaned dairy calves in the Czech Republic: Absence of C. ryanae and management-associated distribution of C. andersoni, C. bovis and C. parvum subtypes
Tvůrce(i)	Kváč, Martin (BC-A)_{RID, RID, ORCID} Hromadová, N. (CZ) Květoňová, Dana (BC-A)_SAI Rost, M. (CZ) Sak, Bohumil (BC-A)_{RID, ORCID}
Zdroj.dok.	Veterinary Parasitology. - : Elsevier - ISSN 0304-4017 Roč. 177, 3/4 (2011), s. 378-382
Poč.str.	5 s.
Jazyk dok.	eng - angličtina
Země vyd.	NL - Nizozemsko
Klíč. slova	Calves ; Cryptosporidium andersoni ; C. bovis ; C. parvum ; GP60 ; SSU
Vědní obor RIV	GJ - Choroby a škůdci zvířat, veterinární medicína
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000290696800026
DOI	10.1016/j.vetpar.2010.11.048
Anotace	A total of 750 faecal samples of dairy calves kept in various housing systems were screened for Cryptosporidium spp. infection using the aniline-carbol-methyl violet staining method.DNAwas extracted from Cryptosporidium positive samples and from 150 randomly selected microscopically negative samples. Nested PCR was performed to amplify the partial SSU rRNA gene that was subsequently digested by SspI, VspI and MboII restriction enzymes. In addition, the C. parvum samples were subsequently analyzed at the GP60 gene to determine the distribution of zoonotic subtypes. Sequence analyses and RFLP identified C. parvum in 137, C. andersoni in 21 and C. bovis in 3 samples. Neither mixed infections nor C. ryanae was detected. IIaA15G2R1 and IIaA16G1R1 were detected as major subtypes. 3 of the 5 detected subtypes were previously associated with human cryptosporidiosis, and moreover, the IIaA15G1R1 subtype, previously reported in humans only, was detected in calves for the first time.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2012

Počet záznamů: 1