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Bioluminescence of Pseudomonas Fluorescens HK44 in the Course of Encapsulation into Silica Gel. Effect of Methanol
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SYSNO ASEP 0359057 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Bioluminescence of Pseudomonas Fluorescens HK44 in the Course of Encapsulation into Silica Gel. Effect of Methanol Tvůrce(i) Trögl, J. (CZ)
Kuncová, Gabriela (UCHP-M) RID, SAI
Kuráň, P. (CZ)Zdroj.dok. Folia Microbiologica. - : Springer - ISSN 0015-5632
Roč. 55, č. 6 (2010), s. 569-575Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova catabolic reporter bacterium ; recombinant escheria-coli ; optical biosensor Vědní obor RIV CE - Biochemie CEP ME 893 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z40720504 - UCHP-M (2005-2011) UT WOS 000286466300004 DOI 10.1007/s12223-010-0091-9 Anotace The bioluminescence (BLM) and colony-forming units (CFU) of Pseudomonas fluorescens HK44 were monitored during encapsulation into pre-polymerized Si(OMe)(4). The non-induced BLM of free cells was increased in the presence of 0.5-2.5 % MeOH. After mixing silica sol with the cell suspension, both BLM and CFU dropped to 1-3 and 8-18 %, respectively; both remained lowered as long as the silica biofilm contained residual MeOH. The kinetics of MeOH being released from silica biofilms (a thickness of 2-6 mm) were first-order. The decrease of bacterial activity due to encapsulation was proportional to the biofilm thickness. MeOH evolving during encapsulation is probably the principal stress factor but not the only one. Pracoviště Ústav chemických procesů Kontakt Eva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227 Rok sběru 2012
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