Počet záznamů: 1

Development of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni

SYSNO ASEP	0358458
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Development of a Multilocus Sequence Tool for Typing Cryptosporidium muris and Cryptosporidium andersoni
Tvůrce(i)	Feng, Y. (CN) Yang, W. (US) Ryan, U. M. (AU) Zhang, L. (CN) Kváč, Martin (BC-A)_{RID, RID, ORCID} Koudela, Břetislav (BC-A)_ORCID Modrý, David (BC-A)_{RID, ORCID} Li, N. (CN) Fayer, R. (US) Xiao, L. (US)
Zdroj.dok.	Journal of Clinical Microbiology - ISSN 0095-1137 Roč. 49, č. 1 (2011), s. 34-41
Poč.str.	8 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	ASTERN UNITED-STATES ; RIBOSOMAL-RNA GENE ; PHYLOGENETIC ANALYSIS ; MOLECULAR ANALYSIS ; NATURAL INFECTION ; DAIRY-CATTLE ; PREVALENCE ; GENOTYPES ; HUMANS ; KENYA
Vědní obor RIV	GJ - Choroby a škůdci zvířat, veterinární medicína
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000285787100003
DOI	10.1128/JCM.01329-10
Anotace	Although widely used for the characterization of the transmission of intestinal Cryptosporidium spp., genotyping tools are not available for C. muris and C. andersoni, 2 of the most common gastric spp. infecting mammals. In this study, we screened the C. muris whole-genome sequencing data - among the 13 potential loci (6 microsatellite and 7 minisatellite loci) evaluated by PCR and DNA sequencing, 4 were chosen. DNA sequence analyses of 44 samples showed the presence of 5 to 10 subtypes of C. muris and 1 to 4 subtypes of C. andersoni at each locus. Altogether, 11 C. muris and 7 C. andersoni multilocus sequence typing (MLST) subtypes were detected among the 16 C. muris and 12 C. andersoni specimens successfully sequenced at all 4 loci. In all analyses, the C. muris isolate (TS03) that originated from an East African mole rat differed significantly from other C. muris isolates, approaching the extent of genetic differences between C. muris and C. andersoni.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2012

Počet záznamů: 1