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The effects of membrane compartmentalization of csk on TCR signaling
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SYSNO ASEP 0358089 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The effects of membrane compartmentalization of csk on TCR signaling Tvůrce(i) Otáhal, Pavel (UMG-J) RID
Pata, Supansa (UMG-J)
Angelisová, Pavla (UMG-J) RID
Hořejší, Václav (UMG-J) RID
Brdička, Tomáš (UMG-J) RIDZdroj.dok. Biochimica et biophysica acta - ISSN 0006-3002
Roč. 1813, č. 2 (2010), s. 367-376Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova T-cell activation ; lipid rafts ; CBP Vědní obor RIV EB - Genetika a molekulární biologie CEP GEMEM/09/E011 GA ČR - Grantová agentura ČR 1M0506 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000287470800010 DOI 10.1016/j.bbamcr.2010.12.003 Anotace The TCR signal transduction is initiated by the activation of Src-family kinases (SFK) which phosphorylate Immunoreceptor tyrosine-based activation motifs (ITAM) present in the intracellular parts of the T-cell receptor (TCR) signaling subunits. Numerous data suggest that after stimulation TCR interacts with membrane rafts and thus it gains access to SFK and other important molecules involved in signal transduction. One of the key questions is how SFK access TCR and what is the importance of non-raft and membrane raft-associated SFK for the initiation and maintenance of the TCR signaling. To answer this question we targeted a negative regulator of SFK, C-terminal Src kinase (Csk) to membrane rafts, recently described "heavy rafts" or non-raft membrane. Our data show that only Csk targeted into "classical" raft but not to "heavy raft" or non-raft membrane effectively inhibits TCR signaling, demonstrating the critical role of membrane raft-associated SFK in this process. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2011
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