Počet záznamů: 1

Pre-sorting endosomal transport of the GPI-anchored protein, CD59, is regulated by EHD1

  1. 1.
    SYSNO ASEP0358085
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    NázevPre-sorting endosomal transport of the GPI-anchored protein, CD59, is regulated by EHD1
    Tvůrce(i)Cai, B. (US) - Katafiasz, D. (US) - Hořejší, Václav (UMG-J) - Naslavsky, N. (US)
    Rozsah stran102 - 120
    Zdroj.dok.Traffic - ISSN 1398-9219
    Poč.str.19 s.
    Jazyk dok.eng - angličtina
    Země vyd.DK - Dánsko
    Klíč. slovacanine kidney cells ; recycling compartment ; receptor
    Vědní obor RIVEB - Genetika a molekulární biologie
    CEZAV0Z50520514 - UMG-J (2005-2011)
    UTISI000285206500010
    DOI10.1111/j.1600-0854.2010.01135.x
    AnotaceThe potential role of EHD1 in regulating the family of glycosylphosphatidylinositol-anchored proteins (GPI-APs) has not been determined. Here we demonstrate a novel role for EHD1 in regulating the trafficking of CD59, an endogenous GPI-AP, at early stages of trafficking through the endocytic pathway. EHD1 displays significant colocalization with newly internalized CD59. Upon EHD1 depletion, there is a rapid Rab5-independent coalescence of CD59 in the ERC region. However, expression of an active Arf6 mutant (Q67L), which traps internalized pre-sorting endosomal cargo in phosphatidylinositol(4,5)-bisphosphate enriched vacuoles, prevents this coalescence. It is of interest that sustained PKC activation leads to a similar coalescence of CD59 at the ERC, and treatment of EHD1-depleted cells with a PKC inhibitor (Go6976) blocked this rapid relocation of CD59. However, unlike sustained PKC activation, EHD1 depletion does not induce the translocation of PKC alpha to ERC.
    PracovištěÚstav molekulární genetiky
    KontaktGabriela Marešová, maresova@img.cas.cz, Tel.: 241 063 217
    Rok sběru2011