Počet záznamů: 1
Dehydrosilybin attenuates the production of ROS in rat cardiomyocyte mitochondria with an uncoupler-like mechanism
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SYSNO ASEP 0355107 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Dehydrosilybin attenuates the production of ROS in rat cardiomyocyte mitochondria with an uncoupler-like mechanism Tvůrce(i) Gabrielová, E. (CZ)
Jabůrek, Martin (FGU-C) ORCID, RID
Gažák, Radek (MBU-M) RID, ORCID
Vostálová, J. (CZ)
Ježek, Jan (FGU-C) RID, ORCID
Křen, Vladimír (MBU-M) RID, ORCID
Modrianský, M. (CZ)Zdroj.dok. Journal of Bioenergetics and Biomembranes - ISSN 0145-479X
Roč. 42, č. 6 (2010), s. 499-509Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Reactive oxygen species ; Cardiomyocytes ; Dehydrosilybin Vědní obor RIV CE - Biochemie CEP GA303/08/0658 GA ČR - Grantová agentura ČR CEZ AV0Z50110509 - FGU-C (2005-2011) AV0Z50200510 - MBU-M (2005-2011) UT WOS 000285753300010 DOI 10.1007/s10863-010-9319-2 Anotace Reactive oxygen species (ROS) originating from mitochondria are perceived as a factor contributing to cell aging. Silybin and dehydrosilybin, two polyphenolic compounds, display a plethora of biological effects generally ascribed to their known antioxidant capacity. When investigating the cytoprotective effects of these two compounds in the primary cell cultures of neonatal rat cardiomyocytes, we noted the ability of dehydrosilybin to de-energize the cells by monitoring JC-1 fluorescence. Experiments evaluating oxygen consumption and membrane potential revealed that dehydrosilybin uncouples the respiration of isolated rat heart mitochondria albeit with a much lower potency than synthetic uncouplers. We infer that the apparent uncoupler-like activity of dehydrosilybin is the basis of its ROS modulation effect in neonatal rat cardiomyocytes and leads us to propose a hypothesis on natural ischemia preconditioning by dietary polyphenols Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2011
Počet záznamů: 1