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Bioluminescent Bioreporter Pseudomonas putida TVA8 as a Detector of Water Pollution. Operational Conditions and Selectivity of Free Cells Sensor
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SYSNO ASEP 0354863 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Bioluminescent Bioreporter Pseudomonas putida TVA8 as a Detector of Water Pollution. Operational Conditions and Selectivity of Free Cells Sensor Tvůrce(i) Kuncová, Gabriela (UCHP-M) RID, SAI
Pazlarová, J. (CZ)
Hlavatá, Alena (UCHP-M)
Ripp, S. (US)
Sayler, G.S. (US)Zdroj.dok. Ecological Indicators. - : Elsevier - ISSN 1470-160X
Roč. 11, č. 3 (2011), s. 882-887Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova whole-cell biosensor ; bioluminiscence ; pseudomonas putida TVA8 Vědní obor RIV EI - Biotechnologie a bionika CEP ME 893 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z40720504 - UCHP-M (2005-2011) UT WOS 000287291100014 DOI 10.1016/j.ecolind.2010.12.001 Anotace Bioluminescence induction was tested in minimal medium. At 15 °C the highest bioluminescence induced with toluene (1.325 mg/L) was reached after 6 h. At 25 °C the bioluminescence maximum was approximately 20% lower but this was reached after 3.5 h, and at temperatures of 7 °C, 28 °C, 30 °C and 34 °C, bioluminescence peaked at ≤60% of the maximum. Time courses of bioluminescence were dependent on cell concentrations. The heights of bioluminescence maxima were proportional to toluene concentration in the range 0–26 mg/L. Twenty-three organic pollutants were tested as bioluminescent inducers. Bioluminescence was also induced with ethanol and methanol and the presence of these alcohols in concentrations of ≤1% increased bioluminescence of toluene. The induction of bioluminescence from samples of wastewater and groundwater contaminated with BTEX from 0.5 to 120 mg/L was demonstrated. Pracoviště Ústav chemických procesů Kontakt Eva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227 Rok sběru 2011
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