Počet záznamů: 1
Stress Proteins in the Cytoplasmic Membrane Fraction of Bacillus subtilis
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SYSNO ASEP 0354589 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Stress Proteins in the Cytoplasmic Membrane Fraction of Bacillus subtilis Tvůrce(i) Petráčková, Denisa (MBU-M) RID, ORCID
Šemberová, L. (CZ)
Halada, Petr (MBU-M) RID, ORCID
Svoboda, Petr (FGU-C) RID, ORCID
Svobodová, J. (CZ)Zdroj.dok. Folia Microbiologica. - : Springer - ISSN 0015-5632
Roč. 55, č. 5 (2010), s. 427-434Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova FUNCTION SIGMA-FACTOR ; SACCHAROMYCES-CEREVISIAE ; IDENTIFICATION Vědní obor RIV EE - Mikrobiologie, virologie CEP LC07017 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50200510 - MBU-M (2005-2011) AV0Z50110509 - FGU-C (2005-2011) UT WOS 000282873200004 DOI 10.1007/s12223-010-0072-z Anotace Stress proteomes of the cytoplasmic membrane fraction of Bacillus subtilis trp (C2)-exposed to acid pH and ethanol were characterized. Although these stress factors impair the cell function in a specific manner, they share the ability to denature proteins. Therefore, specific and general stress proteins in the membranes were investigated. Both ethanol (3 percent) and pH 5.0 increase the doubling time from 17 to 25 min. Isolated cytoplasmic membranes were subjected to an optimized 2D PAGE analysis which permitted the separation and analysis of 450 distinct protein spots. Two alternative methods of protein detection were compared, i.e. silver staining and (35)S-L-methionine pulse labeling; the stress induced proteins were identified by MALDI-TOF MS. After ethanol stress, five proteins were increased, viz. YdaP, Ctc, YfhM, YjcH and YwaC. Acid stress proteins were AcoB, YkwC, SodA, YjcH and YwaC. Proteins YjcH and YwaC were increased after ethanol as well as acid pH treatment Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2011
Počet záznamů: 1