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Development of soil microbiology methods: from respirometry to molecular approaches
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SYSNO ASEP 0354462 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Development of soil microbiology methods: from respirometry to molecular approaches Tvůrce(i) Gabriel, Jiří (MBU-M) RID Zdroj.dok. Journal of Industrial Microbiology & Biotechnology - ISSN 1367-5435
Roč. 37, č. 12 (2010), s. 1289-1297Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Soil microbiology ; Continuous cultivation ; Microbial growth Vědní obor RIV EE - Mikrobiologie, virologie CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000284373300010 DOI 10.1007/s10295-010-0866-7 Anotace This review deals with techniques and methods used in the study of the function and development of microorganisms occurring in soil with emphasis on the contributions of Czech Academician Ivan Malek and his coworkers or fellows (JiA (TM) i Macura, Frantiek Kunc) to the development of basic techniques used in soil microbiology. Early studies, including batch cultivation and respirometric techniques, as well as later developments of percolation and continuous-flow methods of cultivation of soil microorganisms are discussed. Recent developments in the application of analytical chemistry (HPLC or GC) and of molecular biological techniques to ecological questions that have revolutionized concepts in soil microbiology and microbial ecology are also briefly mentioned, including denaturing gradient gel electrophoresis (DGGE), temperature gradient gel electrophoresis (TGGE), phospholipid fatty acid analysis (PLFA) and others Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2011
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