Počet záznamů: 1

Yeast 18 S rRNA Is Directly Involved in the Ribosomal Response to Stringent AUG Selection during Translation Initiation

SYSNO ASEP	0354392
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Yeast 18 S rRNA Is Directly Involved in the Ribosomal Response to Stringent AUG Selection during Translation Initiation
Tvůrce(i)	Nemoto, N. (US) Singh, Ch. R. (US) Udagawa, T. (US) Wang, S. (US) Thorson, E. (US) Winter, Z. (US) Ohira, T. (US) Li, M. (US) Valášek, Leoš (MBU-M)_RID Brown, S. J. (US) Asano, K. (US)
Zdroj.dok.	Journal of Biological Chemistry. - : Elsevier - ISSN 0021-9258 Roč. 285, č. 42 (2010), s. 32200-32212
Poč.str.	13 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	START CODON SELECTION ; SACCHAROMYCES-CEREVISIAE ; IN-VIVO
Vědní obor RIV	EE - Mikrobiologie, virologie
CEZ	AV0Z50200510 - MBU-M (2005-2011)
UT WOS	000282683000033
DOI	10.1074/jbc.M110.146662
Anotace	The C-terminal domain (CTD) of the a/Tif32 subunit of budding yeast eukaryotic translation initiation factor 3(eIF3) interacts with eIF3 subunits j/Hcr1 and b/Prt1 and can bind helices 16 to 18 of 18S rRNA, suggesting proximity to the mRNA entry channel of the 40S subunit. We have identified substitutions in the conserved Lys-Glu-Arg-Arg (KERR) motif and in residues of the nearby box6 element of the a/Tif32 CTD that impair mRNA recruitment by 43S preinitiation complexes (PICs) and confer phenotypes indicating defects in scanning and start codon recognition. The normally dispensable CTD of j/Hcr1 is required for its binding to a/Tif32 and to mitigate the growth defects of these a/Tif32 mutants, indicating physical and functional interactions between these two domains
Pracoviště	Mikrobiologický ústav
Kontakt	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Rok sběru	2011

Počet záznamů: 1